SKU: ES855 | Special Order Item: No | Medical Device: No
Staining of bacteria from cultures or patient specimens suspected to contain Mycobacteria tuberculosis can be easily achieved using one of two methods: (1) hot method or (2) cold method. The traditional Ziehl Neelsen Method employs the use of heat, for which it is known as the hot method. Ziehl Neelsen staining method uses heated Carbol-fuchsin solution as a primary stain, followed by a decolorizer solution and a methylene blue solution as a counterstain. Whereas the Kinyoun Method provides a cold method alternative for staining without the need for heat with brilliant green or methylene blue counterstain option.
Acid-fast organisms are stained red, while non-acid-fast organisms and the background are stained green or blue. A major characteristic of mycobacteria is that they are acid-fast and once stained with aniline dye, basic fuchsin, they are difficult to decolorize, thus retaining the red color even when exposed to an acid alcohol rinse. Whether youre testing surgical specimens, respiratory or sputum specimens, the two counterstains brilliant green and methylene blue will provide easy identification to the non-acid fast organisms.
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