SKU: ES37263 | Special Order Item: No | Medical Device: No
Wheatleys Trichrome Stain technique for fecal specimens is a modification of the Gomori staining procedure for tissue. Wheatleys modification provides a rapid procedure that produces uniform, well-stained smears of intestinal protozoa, human cells, yeast cells, and artifact material. It is known to be the best stain to support stool examinations for intestinal protozoa.
Features:
– 8 oz. bottles with spout caps
Specimen Notes:
1. Dry PVA fixed smears for 1 hour at 35-37°C or overnight at room temperature prior to staining.
2. Do not allow Schaudinns fixed smears to dry out at any time during the procedure.
3. Fecal smears prepared from SAF-preserved stool material do not require the iodine step.
4. If the smears fall off slide, the specimen might have been inadequately dried on the slide (particularly in the case of PVA-fixed specimens) or the slides may have an oily film residue
Staining Procedure:
Smears prepared from PVA and Schaudinns fixed material must proceed through an Iodine Alcohol Rinse to remove mercuric chloride. If slides are produced from SAF preserved or mercuric chloride free fixative, skip to Step 2.
1. Prepare Acid Alcohol, 0(more…)
