SKU: ES4808 | Special Order Item: No | Medical Device: No
The Fites Stain Kit (For Leprosy and Nocardia) is intended for use in the histological visualization of mycobacterium Lepra bacillus and Nocardia. This kit may be used on formalin-fixed, paraffin-embedded or frozen sections.
Lerpa bacillus: Red
Nocardia: Red
Background: Blue
Uses/Limitations:
– For In-Vitro Diagnostic use only
– Histological applications
– Do not use past expiration date
– Use caution when handling these reagents
Control Tissue: Any well fixed paraffin embedded tissue
Lepra bacillus Procedure (Standard):
1. Deparaffinize sections in 2 changes of Xylene-Peanut Oil Solution for 12 minutes each.
2. Air dry slide for 15 minutes. Do not remove oil film. Remaining film prevents de-staining of Lepra bacillus during differentiation.
3. Rinse slide in several changes of distilled water.
4. Incubate slide in Carbol Fuchsin Solution for 15 minutes.
5. Rinse slide in several changes of distilled water.
6. Differentiate section in Acid Alcohol Solution (1%) until background is pink.
7. Rinse slide in distilled water and check by microscope for correct differentiation
8. Rinse in running tap water for 1 minute fo(more…)
