SKU: ES4807 | Special Order Item: No | Medical Device: No
The Elastic Stain Kit (Modified Verhoff’s) is intended for use in the histological demonstration of elastin in tissue sections. Demonstration of elastic tissue is useful in cases of emphysema (atrophy of elastic tissue), arteriosclerosis (thinning and loss of elastic fibers) and various other vascular diseases.
Elastic Fibers: Black to Blue/Black
Nuclei: Blue to Black
Collagen: Red
Muscle & Other: Yellow
Uses/Limitations:
– For In-Vitro Diagnostic use only
– Histological applications
– Do not use past expiration date
– Use caution when handling these reagents
Control Tissue: Lung or any vascular tissue
Preparation of Reagents Prior to Beginning:
1. Prepare working Elastic Stain Solution by mixing 30ml Hematoxylin Solution (5%) with 12ml Ferric Chloride
Solution (10%) and 12ml Lugols Iodine Solution. Mixed solution may be used for 24 hours.
Note: Removal of mercury deposits is not required for tissues that have been fixed in mercury containing fixatives
since it will be removed by the staining solution.
Procedure:
1. Deparaffinize sections if necessary and hydrate to disti(more…)
