SKU: ES4817 | Special Order Item: No | Medical Device: No
The P.T.A.H. Stain Kit for Microwave is intended for use in the histological visualization of collagen, striated muscle and glial fibers without using Zenkers Fixative with Mercuric Chloride as a mordant. This kit may be used on formalin-fixed, paraffin-embedded, or frozen sections.
Fibrin, Striated Muscle, Glial Fibers: Blue
Collagen: Brownish/Red
Nuclei: Blue
Uses/Limitations:
– For In-Vitro Diagnostic use only
– Histological applications
– Do not use past expiration date
– Use caution when handling these reagents
Control Tissue: Striated Muscle
Equipment Needed: 500 Watt Microwave Oven
Procedure:
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Place slide to fresh distilled water for 1 minute.
3. Pour 50 ml of Zinc Chloride Solution (10%) into plastic coplin jar and heat in microwave for 20 seconds on high power. Remove jar and stir solution to equalize temperature. Return coplin jar to microwave and heat for 10 seconds on high power. Remove jar and stir solution to equalize temperature.
4. Place slide in coplin jar and incubate for 15 minutes.
5. Rinse slide in running tap water fo(more…)
