SKU: ES4810 | Special Order Item: No | Medical Device: No
The Hepatitis B Stain Kit is intended for use in the histological visualization of Hepatitis B surface antigen (HBsAg). This kit may be used on formalin-fixed, paraffin-embedded or frozen sections.
HBsAg: Dark Brown/Purple
Uses/Limitations:
– For In-Vitro Diagnostic use only
– Histological applications
– Do not use past expiration date
– Use caution when handling these reagents
Control Tissue: Any well fixed section cut 3-5 microns
Prepare Oxidizing Immediately Prior to Beginning Procedure:
Combine:
50 ml Distilled Water
5 ml Potassium Permanganate Solution (5%)
3 ml Sulfuric Acid Solution (3%)
Mix thoroughly.
Procedure:
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Incubate slide in freshly prepared Oxidizing Solution for 10 minutes.
3. Rinse slide briefly in running tap water followed by 1 dip in distilled water.
4. Incubate slide in Oxalic Acid Solution (2%) for 10 minutes.
Note: Section should be colorless following this step.
5. Rinse slide for 1 minute in running tap water followed by 2 dips in distilled water.
6. Incubate slide in Orcein Solution for 2 hours.
7. Rinse sli(more…)
