SKU: ES4811 | Special Order Item: No | Medical Device: No
The H. Pylori Rapid Stain Kit is intended for demonstrating Helicobacter Pylori infected tissue. Kit may be used on formalin fixed, paraffin-embedded tissue, or frozen sections.
Helicobacter Pylori: Blue
Mucin: Yellow
Background: Light Blue
Uses/Limitations:
– For In-Vitro Diagnostic use only
– Histological applications
– Do not use past expiration date
– Use caution when handling these reagents
Control Tissue: Helicobacter Pylori infected stomach
Prepare Toluidine Blue Working Solution (Immediately prior to use):
Distilled Water 50 ml
Toluidine Blue Solution 20 drops
Sodium Hydroxide Solution 3 drops
Procedure (Standard):
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Incubate slide in Periodic Acid Solution for 10 minutes (Note: Do Not Reuse Periodic Acid Solution).
3. Rinse thoroughly in distilled water.
4. Incubate slide in Sodium Metabisulfite Solution for 5 minutes.
5. Rinse thoroughly in distilled water.
6. Incubate slide in Alcian Yellow Solution for 5-10 minutes.
7. Rinse slide thoroughly in distilled water.
8. Incubate slide in freshly prepared Toluidine Bl(more…)
