SKU: ES3404 | Special Order Item: No | Medical Device: No
The Gram Stain Kit is intended for the demonstration and differentiation of Gram-positive and Gram-negative bacteria.
Gram Positive Bacteria: Blue
Gram Negative Bacteria: Red
Other Tissue: Yellow
Nuclei: Red
Uses/Limitations:
– For In-Vitro Diagnostic use only
– Histological applications
– Do not use past expiration date
– Use caution when handling these reagents
Control Tissue: Any well fixed paraffin embedded tissue section
Procedure (Standard):
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Flood slide with Gentian Violet Solution and incubate for 1 minute.
3. Rinse slide in distilled water to remove excess stain.
4. Flood slide with Lugols Iodine Solution and incubate for 1 minute.
5. Rinse slide in running tap water to remove excess Iodine.
6. Place slide in Grams Decolorizer until color no longer bleeds off section.
7. Rinse slide quickly in running tap water.
8. Flood slide with Carbol Fuchsin and incubate for 1-2 minutes.
9. Rinse slide quickly in running tap water to remove excess stain.
10. Apply Tartrazine Sol(more…)
