SKU: ES4814 | Special Order Item: No | Medical Device: No
The Oil Red O Stain Kit (For Fat) is intended for use in the histological visualization of fat cells and neutral fat. This kit may be used ONLY on frozen tissue sections, fresh smears, or touch preps as xylenes and alcohols will dissolve fat deposits.
Fat Cells: Red
Neutral Fat: Red
Nuclei: Blue
Uses/Limitations:
– For In-Vitro Diagnostic use only
– Histological applications
– Do not use past expiration date
Control Tissue: Any frozen section containing fat
Procedure (Standard):
Note: Heat Oil Red O Solution to 60°C prior to beginning.
1. Prepare fresh or frozen tissue section as usual.
2. Place slide in Propylene Glycol for 2 minutes.
3. Incubate slide in heated Oil Red O Solution for 6-10 minutes or overnight at room temperature.
Note: Prepare mixture of 85% Propylene Glycol in distilled water.
4. Differentiate tissue section in 85% Propylene Glycol for 1 minute.
5. Rinse slide in 2 changes of distilled water.
6. Incubate slide in Hematoxylin, Mayers (Lillies Modification) for 1-2 minutes.
7. Rinse slide thoroughly in tap water.
8. Rinse slide in 2 changes of distilled water.
9. Coverslip u(more…)
